|本期目录/Table of Contents|

[1]宋翔,谢希贤,徐庆阳,等.苹果酸对L-谷氨酸发酵代谢流迁移的影响[J].生物加工过程,2009,7(05):58-63.
 SONG Xiang,XIE Xi-xian,XU Qing-yang,et al.Effects of malate on metabolic flux transfer of L-glutamic acid fermentation[J].Chinese Journal of Bioprocess Engineering,2009,7(05):58-63.
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苹果酸对L-谷氨酸发酵代谢流迁移的影响
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
7
期数:
2009年05期
页码:
58-63
栏目:
出版日期:
2009-09-30

文章信息/Info

Title:
Effects of malate on metabolic flux transfer of L-glutamic acid fermentation
文章编号:
1672-3678(2009)05-0058-06
作者:
宋翔谢希贤徐庆阳刘淑云陈宁
天津科技大学 生物工程学院 工业微生物教育部重点实验室,天津300457
Author(s):
SONG Xiang XIE Xi-xian XU Qing-yang LIU Shu-yun CHEN Ning
Key Laboratory of Industrial Microbiology of the Ministry of Education,College of Biotechnology,
Tianjin University of Science and Technology,Tianjin 300457, China
关键词:
黄色短杆菌L-谷氨酸' target="_blank" rel="external">">L-谷氨酸线性规划代谢流分析
分类号:
Q93
文献标志码:
A
摘要:
为更全面深入地理解细胞内谷氨酸代谢的调控机制,以黄色短杆菌GDK-9为供试菌株,应用MATLAB软件和代谢流分析方法定量研究添加苹果酸后L-谷氨酸发酵中、后期胞内的代谢流迁移。在L-谷氨酸发酵中、后期添加 2.0 g/L苹果酸后,合成副产物L-丙氨酸和乳酸的代谢流量明显减少,分别降低了22.1%和16.5%,EMP途径和乙醛酸循环的代谢流分别减少了2.26%和9.09%,HMP途径的代谢流增加了2.26%,而L-谷氨酸生物合成的代谢流从73.59%增长至79.92%,较未添加前提高了6.33%。添加适量苹果酸能使关键节点发生代谢流迁移,提高了L-谷氨酸合成中心代谢途径的代谢流量。

参考文献/References:

[1]张蓓.代谢工程[M].天津:天津大学出版社,2003:149-154.
[2]陈宁.氨基酸工艺学[M].北京:中国轻工业出版社,2007:77-83.
[3]王峥,郝宁,许琳,等.谷氨酸棒杆菌生物合成L-鸟氨酸的代谢流分析[J].南京工业大学学报:自然科学版,2009,31(4):36-40.
Wang Zheng, Hao Ning, Xu Lin, et al. Metabolic flux analysis of L-ornithine biosynthesis by Corynebacterium glutamicum[J]. Journal of Nanjing University of Technology: Natural Science Edition,2009,31(4):36-40.
[4]Schuster S, Klamt S, Weckwerth W, et al.Use of network analysis of metabolic systems in bioengineering[J].Bio-Process Biosys Eng,2002, 24: 363-372.
[5]Goel A, Ferrance J, Jeong J,et al.Analysis of metabolic fluxes in batch and continuous cultures of Bacillus subtilis[J].Biotechnol Bioeng, 1993, 42(4): 686-696.
[6]Vallino J J, Stephanopoulos G. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction[J].Biotechnol Bioeng, 1993, 41(6): 633-646.
[7]Kimura E. Metabolic engineering of glutamate production[M]∥Advances in Biochemical Engineering/Bioteehrology. Berlin/Heidelberg: Springer,2003:37-57.
[8]Shimizu H, Hirasawa T. Molecular mechanism analysis and metabolic Engineering[M]∥Amino Acid Biosynthesis.Berlin/Heidelberg: Springer,2007:1-38.
[9]Takac S, Calik G, Mavituna F, et al. Metabolic flux distribution for the optimized production of L-glutamate[J]. Enzyme and Microbial Technology,1998,23(5):286-300.
[10]Pierre Gourdon, Nicholas D. Lindley. Metabolic analysis of glutamate production by Corynebacterium glutamicum[J]. Metabolic Engineering, 1999,1, 224-231.
[11]杜军,陈宁,刘辉,等.基于代谢流量分析的L-谷氨酸发酵过程优化[J].中国食品报,2008,8(2):30-35.
Du Jun, Chen Ning, Liu Hui, et al. Application of metabolic flux analysis for optimization of L-glutamic acid fermentation process [J]. Journal of Chinese Institute of Food Science and Technology,2008,8 (2):30-35.
[12]杜军,刘辉,徐庆阳,等.基于途径分析的L-谷氨酸发酵条件优化[J].食品与发酵工业,2007,33(11):9-12.
Du Jun, Liu Hui, Xu Qingyang,et al. Application of pathway analysis for optimization of L-glutamic acid production[J]. Food and Fermentation Industries,2007,33(11):9-12.
[13]陈宁,刘辉.柠檬酸钠酯对L-亮氨酸发酵代谢流分布的影响[J].高校化学工程学报,2008,22(3):478-483.
Chen Ning, Liu Hui. Effects of sodium citrate on metabolic fludistributions of L-leucine production by Brevibacterium flavum TK0303 [J]. Journal of Chemical Engineering of Chinese Universities,2008,22(3):478-483.
[14]Majewski R A, Domach M M.Simple constrained optimization view of acetate overflow in E.coli[J]. Biotechnol Bioeng,1990, 35(7): 732-738.

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备注/Memo

备注/Memo:
收稿日期:2008-11-03
基金项目:国家科技支撑计划资助项目(2007BAK36B00);国家高技术研究发展计划(863计划)资助项目(2006AA020301);天津科技大学引进人才科研启动基金资助项目(20070441)
作者简介:宋翔(1984—),男,山西太原人,硕士研究生,研究方向:代谢工程;陈宁(联系人),教授,E-mail: ningch@tust.edu.cn
更新日期/Last Update: