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[1]霍光明,张李阳,朱枳穆,等.响应面法优化链霉菌S10A09发酵产纤维素酶条件[J].生物加工过程,2019,17(02):138-143.[doi:10.3969/j.issn.1672-3678.2019.02.004]
 HUO Guangming,ZHANG Liyang,ZHU Zhimu,et al.Optimization of Streptomyces sp. S10A09 for cellulase production by response surface method[J].Chinese Journal of Bioprocess Engineering,2019,17(02):138-143.[doi:10.3969/j.issn.1672-3678.2019.02.004]
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响应面法优化链霉菌S10A09发酵产纤维素酶条件()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年02期
页码:
138-143
栏目:
出版日期:
2019-03-30

文章信息/Info

Title:
Optimization of Streptomyces sp. S10A09 for cellulase production by response surface method
文章编号:
1672-3678(2019)02-0138-06
作者:
霍光明12张李阳12朱枳穆1俞丽娜1
1.南京晓庄学院 食品科学学院,江苏 南京 211171; 2.江苏省高校“特殊生物质废弃物资源化利用”重点建设实验室,江苏 南京 211171
Author(s):
HUO Guangming12ZHANG Liyang12ZHU Zhimu1YU Lina1
1.School of Food Science,Nanjing Xiaozhuang University,Nanjing 211171,China; 2.Jiangsu Provincial Key Construction Laboratory of Special Biomass Waste Resource Utilization,Nanjing 211171,China
关键词:
纤维素酶 Plackett-Burman 响应面法 滤纸酶活 链霉菌
分类号:
Q812
DOI:
10.3969/j.issn.1672-3678.2019.02.004
文献标志码:
A
摘要:
在筛选纤维素酶活菌株时,发现一株放线菌链霉菌属S10A09具有较高的纤维素酶活力。为了获得高酶活纤维素酶,将Plackett-Burman(PB)筛选和中心组合设计(CCD)以及响应面分析法相结合,考察影响链霉菌属S10A09发酵生产滤纸酶的发酵条件。Plackett-Burman结果表明,羧甲基纤维素钠(CMC-Na)和(NH4)2SO4是影响S10A09发酵产纤维素酶活高低的主要因素。CCD实验优化后产酶最优发酵培养基(g/L)为CMC-Na 2.57、(NH4)2SO411.31、KH2PO4 0.2、MgSO41、FeSO40.01。优化后,滤纸酶活(FPA)达到125.96 U/mL,接近优化前的3倍。

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备注/Memo

备注/Memo:
收稿日期:2017-04-05修回日期:2018-06-29
基金项目:江苏省农业三新工程项目(SXGC[2016]022); 江苏省高校重点建设实验室项目(苏教科[2016]8)
作者简介:霍光明(1980—),男,山东蒙阴人,高级实验师,研究方向:微生物发酵,E-mail:cnhuogm@163.com
引文格式:霍光明,张李阳,朱枳穆,等.响应面法优化链霉菌S10A09发酵产纤维素酶条件[J].生物加工过程,2019,17(2):138-143.
HUO Guangming,ZHANG Liyang,ZHU Zhimu,et al.Optimization of Streptomyces sp.S10A09 for cellulase production by response surface method[J].Chin J Bioprocess Eng,2019,17(2):138-143..
更新日期/Last Update: 2019-03-30