|本期目录/Table of Contents|

[1]王向栋,李会,陈志蔚,等.分枝杆菌LY-1转化植物甾醇产9α羟基雄烯二酮的发酵工艺优化[J].生物加工过程,2019,17(02):177-183.[doi:10.3969/j.issn.1672-3678.2019.02.010]
 WANG Xiangdong,LI Hui,CHEN Zhiwei,et al.Fermentation process of Mycobacterium sp. LY-1 for the transformation of phytosterol to 9α-hydroxy-4-androstenedione[J].Chinese Journal of Bioprocess Engineering,2019,17(02):177-183.[doi:10.3969/j.issn.1672-3678.2019.02.010]
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分枝杆菌LY-1转化植物甾醇产9α羟基雄烯二酮的发酵工艺优化()
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《生物加工过程》[ISSN:1672-3678/CN:32-1706/Q]

卷:
17
期数:
2019年02期
页码:
177-183
栏目:
出版日期:
2019-03-30

文章信息/Info

Title:
Fermentation process of Mycobacterium sp. LY-1 for the transformation of phytosterol to 9α-hydroxy-4-androstenedione
文章编号:
1672-3678(2019)02-0177-07
作者:
王向栋1李会1陈志蔚2蔡兆培2史劲松1许正宏3
1. 江南大学 药学院,江苏 无锡 214122; 2. 大丰市佳丰油脂有限责任公司,江苏 盐城 224100; 3.江南大学 粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122
Author(s):
WANG Xiangdong1LI Hui1CHEN Zhiwei2CAI Zhaopei2SHI Jinsong1XU Zhenghong3
1. School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China; 2. Dafeng Jiafeng Oil Co.,Ltd.,Yancheng 224100,China; 3. National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China
关键词:
Mycobacterium sp. 植物甾醇 9α-OH-AD 正交优化
分类号:
Q93
DOI:
10.3969/j.issn.1672-3678.2019.02.010
文献标志码:
A
摘要:
9α-羟基雄甾-4-烯-3,17-二酮(9α-OH-AD)是一种重要的甾体药物中间体,可用于糖皮质激素类药物的生产。为了提高目的产物的产量,以分枝杆菌(Mycobacterium sp.)LY-1作为出发菌株,研究不同碳源、氮源、磷酸盐和金属离子等培养基组分对菌株LY-1转化植物甾醇生产9α-OH-AD的影响,结合正交试验优化,确定最适培养基组分为玉米浆30 g/L、KNO3 4.0 g/L、NaH2PO4 1.2 g/L、FeSO4 0.075 g/L及CaCl2 0.10 g/L。在此条件下,当植物甾醇投料质量浓度为15 g/L时,产物得率达到43.9%~44.9%。通过单因素实验优化培养条件,得到最适培养条件为pH 8.0、接种量2%、温度30 ℃时,9α-OH-AD的得率可达46.2%~47.0%,比优化前提高了22.1%,产量达到5.21 g/L,具有潜在的工业应用价值。

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备注/Memo

备注/Memo:
收稿日期:2018-03-14修回日期:2019-01-14
基金项目:江苏省普通高校学术学位研究生科研创新计划(KYLX16_0826)
作者简介:王向栋(1993—),男,浙江东阳人,硕士研究生,研究方向:微生物与生化药学; 李会(联系人),副教授,E-mail:lihui@jiangnan.edu.cn
引文格式:王向栋,李会,陈志蔚,等.分枝杆菌LY-1转化植物甾醇产9α羟基雄烯二酮的发酵工艺优化[J].生物加工过程,2019,17(2):177-183.
WANG Xiangdong,LI Hui,CHEN Zhiwei,et al.Fermentation process of Mycobacterium sp. LY-1 for the transformation of phytosterol to 9α-hydroxy-4-androstenedione [J].Chin J Bioprocess Eng,2019,17(2):177-183..
更新日期/Last Update: 2019-03-30